| EPR study on the photosensitized generation of reactive oxygen species by actinomycin D |
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| 作者姓名： | 潘景喜 张素萍 屠铁成 韩镇辉 蔡喜臣 姚思德 林念芸 |
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| 作者单位： | PAN Jingxi ZHANG Suping TU TiechengHAN Zhenhui，CAI Xichen，YAO Side& LIN Nianyun1. Laboratory of Radiation Chemistry，Shanghai Institute of Nuclear Research，Chinese Academy of Sciences，Shanghai 201800，China;2. Institute |
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| 摘 要： | Actinomycin D (AMD) is an anticancer antibiotic that can bind selectively to both double-stranded and single-stranded DNA, and this binding greatly enhances DNA photosensitization. Using electron paramagnetic resonance (EPR) in combination with spin trapping techniques, a systematic study was carried out on the reactive oxygen species generated in the photosensitization process of AMD. It was found that 1O2 and O2- are important reactive intermediates either insolution or in DNA complexes, and the generation of these species is in competition. This finding suggests that the photodynamic action of AMD proceeds via two pathways: energy transfer (type Ⅰ mechanism) and electron transfer (type Ⅱ mechanism). 1O2 is the main product formed via energy transfer reaction in solution while electron transfer between the excited states of AMD and DNA becomes the predominant pathway in DNA complexes.
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| 收稿时间： | 03 November 2007 |
EPR study on the photosensitized generation of reactive oxygen species by actinomycin D |
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| Jingxi Pan, Suping Zhang, Tiecheng Tu, Zhenhui Han, Xichen Cai, Side Yao and Nianyun Lin.EPR study on the photosensitized generation of reactive oxygen species by actinomycin D[J].Science in China(Chemistry),2002,45(5):449-454. |
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| Authors: | Jingxi Pan Suping Zhang Tiecheng Tu Zhenhui Han Xichen Cai Side Yao and Nianyun Lin |
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| Institution: | 1. Laboratory of Radiation Chemistry, Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, Shanghai 201800, China
2. Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032, China |
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| Abstract: | Actinomycin D (AMD) is an anticancer antibiotic that can bind selectively to both double-stranded and single-stranded DNA, and this binding greatly enhances DNA photosensitization. Using electron paramagnetic resonance (EPR) in combination with spin trapping techniques, a systematic study was carried out on the reactive oxygen species generated in the photosensitization process of AMD. It was found that 1O2 and O2- are important reactive intermediates either insolution or in DNA complexes, and the generation of these species is in competition. This finding suggests that the photodynamic action of AMD proceeds via two pathways: energy transfer (type I mechanism) and electron transfer (type II mechanism). 1O2 is the main product formed via energy transfer reaction in solution while electron transfer between the excited states of AMD and DNA becomes the predominant pathway in DNA complexes. |
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| Keywords: | actinomycin D EPR reactive oxygen species photodynamic action |
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