| 超高效液相色谱法测定乳及乳制品中的三聚氰胺 |
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| 引用本文： | 王炼,庞晶晶,余辉菊. 超高效液相色谱法测定乳及乳制品中的三聚氰胺[J]. 分析试验室, 2010, 29(4) |
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| 作者姓名： | 王炼 庞晶晶 余辉菊 |
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| 作者单位： | 四川大学华西公共卫生学院,成都,610041;成都市疾病预防控制中心,成都,610041;四川大学华西公共卫生学院,成都,610041;成都市疾病预防控制中心,成都,610041 |
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| 基金项目： | 四川省卫生厅2007年科研(070326);;四川省医学重点实验室项目资助 |
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| 摘 要： | 建立了超高效液相色谱测定乳及乳制品中三聚氰胺的方法。样品经三氯乙酸超声提取,乙酸铅沉淀蛋白,Cleanert PCX固相萃取小柱净化,氨化甲醇洗脱后氮气吹干,流动相溶解后,采用亲水相互作用色谱柱-超高效液相色谱-二极管阵列检测器检测。实验对样品提取和色谱分离条件进行了优化。0.3、1和3μg/g3个质量分数的奶粉加标回收试验,平均回收率和相对标准偏差为分别为66.4%～87.4%和11%～17%,定量下限0.5μg/g。方法能满足乳及乳制品中的快速检测要求,用于实际乳及乳制品的检测获得了满意的结果。
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| 关 键 词： | 三聚氰胺 超高效液相色谱法 乳及乳制品 |
Determination of melamine in the raw milk and dairy products by UPLC |
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| WANG Lian,PANG Jing-jing,YU Hui-ju. Determination of melamine in the raw milk and dairy products by UPLC[J]. Chinese Journal of Analysis Laboratory, 2010, 29(4) |
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| Authors: | WANG Lian PANG Jing-jing YU Hui-ju |
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| Affiliation: | 1.West China School of Public Health;Sichuan University;Chengdu 610041;2.Chengdu Center for Disease Control and Prevention;Chengdu 610041 |
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| Abstract: | An approach for the determination of melamine in the raw milk and dairy products using hydrophilic interaction liquid chromatographic column-ultra performance liquid chromatography with a photodiode array detector is described.The sample was extracted using trichloroacetic acid solution and the protein of samples were precipitated by Pb(Ac)2.The supernatant was cleaned-up with PCX cartridge.The conditions of sample extraction and chromatographic separation were optimized.The average spiked recoveries of sam... |
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| Keywords: | Melamine UPLC Milk |
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