| 抗体和寡核苷酸双标记纳米金生物探针的制备及生物学特性 |
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| 引用本文： | 李富荣,乔飞燕,孔小丽,周汉新,齐晖,任莉莉. 抗体和寡核苷酸双标记纳米金生物探针的制备及生物学特性[J]. 高等学校化学学报, 2009, 30(1): 60-63 |
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| 作者姓名： | 李富荣 乔飞燕 孔小丽 周汉新 齐晖 任莉莉 |
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| 作者单位： | 暨南大学第二临床医学院(深圳人民医院)临床医学研究中心,深圳,518020;兰州大学化学化工学院,兰州,730000 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，深圳市科技局计划项目 |
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| 摘 要： | 纳米金通过静电吸附抗体, 与寡核苷酸共价结合制备双标记纳米金生物探针, 比较了双标记纳米金生物探针和单标记抗体IgG或ss-DNA的稳定性和反应性. 结果表明, 在水溶液中纳米金由于ss-DNA的结合使IgG抗体的吸附能力明显改善, IgG的吸附也影响二硫苏糖醇(DDT)对ss-DNA的解离作用. 双标记纳米粒上覆盖(50±15)条ss-DNA和(10±2)条IgG, 较单标记ss-DNA纳米金上的(70±15)条要少. 斑点免疫和杂交实验证明, 纳米金表面标记的IgG和ss-DNA具有良好生物学活性. 双标记纳米金生物探针在超微量蛋白质的检测中具有应用价值.
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| 关 键 词： | 金纳米粒子 生物探针 寡核苷酸 抗体 |
| 收稿时间： | 2008-04-23 |
Preparation and Biological Characterization of Antibody and DNA Dual-labeled Gold Nanoparticles |
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| LI Fu-Rong,QIAO Fei-Yan,KONG Xiao-Li,ZHOU Han-Xin,QI Hui,REN Li-Li. Preparation and Biological Characterization of Antibody and DNA Dual-labeled Gold Nanoparticles[J]. Chemical Research In Chinese Universities, 2009, 30(1): 60-63 |
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| Authors: | LI Fu-Rong QIAO Fei-Yan KONG Xiao-Li ZHOU Han-Xin QI Hui REN Li-Li |
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| Affiliation: | Clinic Medical Research Center, The 2nd Clinic Medical College(Shenzhen People’s Hospital), Jinan University, Shenzhen 518020, China; ;Department of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China |
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| Abstract: | Gold nanoparticles labeled by both antibody(IgG) and single stranded DNA(ss-DNA) were synthesized and characterized. The stability and reactivity of the dual-labeled nanoparticles were compared with the conventional IgG or ss-DNA modified nanoparticles. It was found that the IgG adsorption significantly improved the stability of the nanoparticles in aqueous solution,which is beneficial for attaching ss-DNA. The presence of IgG also effectively prohibits the desorption of ss-DNA against dithiothreitol(DTT) d... |
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| Keywords: | Gold nanoparticle Bio-probes DNA Antibody |
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