| 酶催化动力学光度法测定抗坏血酸 |
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| 引用本文： | 张建夫,陈亚红,田丰收,邓聪.酶催化动力学光度法测定抗坏血酸[J].光谱实验室,2010,27(1):213-216. |
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| 作者姓名： | 张建夫 陈亚红 田丰收 邓聪 |
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| 作者单位： | 周口师范学院化学系河南省周口市七一路东段31号,466000 |
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| 基金项目： | 河南省高校科技创新人才支持计划资助(No.2009HASTIT034);;河南省教育厅自然科学基金资助项目(No.2008A150029) |
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| 摘 要： | 基于抗坏血酸对血红蛋白模拟酶催化体系的抑制作用,建立酶催化动力学光度法测定抗坏血酸的新方法。研究了体系的最佳条件及动力学行为,测定的线性范围为2.0×10-7—5.9×10-5mol/L,方法检出限为7.0×10-8mol/L。对6.0×10-6mol/L的抗坏血酸进行11次平行测定相对标准偏差为2.5%。此方法可用于药剂和蔬菜中抗坏血酸含量的测定。
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| 关 键 词： | 抗坏血酸 酶催化动力学光度法 血红蛋白 |
Determination of Ascorbic Acid by Enzymatic Catal ytic Spectrophotometry |
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| ZHANG Jian-Fu,CHEN Ya-Hong,TIAN Feng-Shou,DENG Cong.Determination of Ascorbic Acid by Enzymatic Catal ytic Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2010,27(1):213-216. |
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| Authors: | ZHANG Jian-Fu CHEN Ya-Hong TIAN Feng-Shou DENG Cong |
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| Institution: | Department of Chemistry;Zhoukou Normal University;Zhoukou;Henan 466000;P.R.China |
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| Abstract: | The spectrophotometry for the determination of ascorbic acid based on the inhibition of hemoglobin for the oxidation of H_2O_2 with acid chrome blue K was developed.The percentage inhibition(Ⅰ%)of system is under the optimal experimental conditions.The calibration curve is linear in the range of 2.0×10~(-7)-5.9×10~(-5)mol/L with the detection limit of 7.0×10~(-8)mol/L.The relative standard derivation is 2.5%at ascorbic acid concentration of 6.0×10~(-6)mol/L(n=11).This method can be used for the determination of ascorbic acid in pharmaceuticals and vegetables with satisfactory results. |
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| Keywords: | Ascorbic Acid Enzymatic Catalytic Spectrophotometry Hemoglobin |
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