Quantum dot-based array for sensitive detection of <Emphasis Type="Italic">Escherichia coli</Emphasis> |
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| Authors: | Nuria Sanvicens Núria Pascual María Teresa Fernández-Argüelles Javier Adrián José Manuel Costa-Fernández Francisco Sánchez-Baeza Alfredo Sanz-Medel M-Pilar Marco |
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| Institution: | 1.Applied Molecular Receptors Group (AMRg),Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC),Barcelona,Spain;2.Applied Molecular Receptors Group (AMRg),CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN),Barcelona,Spain;3.Department of Physical and Analytical Chemistry,University of Oviedo,Oviedo,Spain;4.Applied Molecular Receptors Group (AMRg), Department of Chemical and Biomolecular Nanotechnology,Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC),Barcelona,Spain |
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| Abstract: | A fluorescent quantum dot-based antibody array, used in sandwich format, has been developed to detect Escherichia coli O157:H7. Numerous parameters such as solid support, optimal concentration of immunoreagents, blocking reagents, and assay
time were optimized for array construction. Quantum dot-conjugated anti-IgG was used as the detecting system. The array allows
the detection of E. coli O157:H7 at concentrations below 10 CFU mL−1 without sample enrichment, exhibiting an increase of three orders of magnitude in the limit of detection compared to ELISA.
The interference caused by Gram (+) and Gram (−) bacteria was negligible at low concentrations of bacteria. |
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