| Abstract: | ![]()
Properties of mixed monolayers of lipid-photosynthetic reaction center proteins (RC) were studied and the optimum conditions for stable films fabrication were determined. The following synthetic: N-acryloylphosphatidylethanolamine (ACPE), tetracosa-11, 13-diinoic acid (TDA), pentacosa-10, 12-diinoic acid (PDA), dioctadecyldienoylphosphatidylcholine (DODL) and natural lipids: L-α-phosphatidylethanolamine (PE), L-α-phosphatidylcholine (PC) were used. The rate of polymerization of the mixed ACPE-RC and TDA-RC monolayers is lower in comparison with corresponding values for pure lipid-like monomers on air/water interface. The optical and photoelectrical measurements provide evidence for an orientation of RCs on interface. Hydrophilic H-subunit in monomeric and polymeric ACPE-RCs, and monomeric DODL-RCs monolayers is preferentially oriented towards water as in the pure RC monolayers. Opposite orientation was found with TDA-RCs and PDA-RCs films. No preferential orientation for lipid-RCs from C. aurantiacus monolayers was found because of the RCs having low assymmetry of hydrophobic subunits (M and L). |
