| 荧光光谱法研究2,4,6-三氯苯酚与胰蛋白酶的相互作用 |
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| 引用本文： | 周秋华,张红梅,文美,王彦卿.荧光光谱法研究2,4,6-三氯苯酚与胰蛋白酶的相互作用[J].理化检验(化学分册),2010(10). |
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| 作者姓名： | 周秋华 张红梅 文美 王彦卿 |
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| 作者单位： | 江苏省滩涂生物资源与环境保护重点建设实验室盐城师范学院应用化学与环境工程研究所; |
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| 基金项目： | 江苏省高校自然科学基础研究项目(09KJD150007); 江苏省“青蓝工程”资助项目 |
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| 摘 要： | 运用荧光光谱法研究了2,4,6-三氯苯酚与胰蛋白酶的相互作用。结果表明:2,4,6-三氯苯酚通过静电和疏水作用力与胰蛋白酶形成基态复合物导致胰蛋白酶内源荧光猝灭,猝灭机理主要为静态猝灭。计算了该反应的表观结合常数K、结合位点数n及结合反应的热力学参数,并用同步荧光和三维荧光技术考察了2,4,6-三氯苯酚对胰蛋白酶构象的影响,酪氨酸和色氨酸残基所处微环境的疏水性增加。
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| 关 键 词： | 荧光光谱 结合反应 2 4 6-三氯苯酚 胰蛋白酶 |
Study on the Interaction of 2,4,6-Trichlorophenol and Trypsin by Fluorescence Spectrometry |
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| ZHOU Qiu-hua,ZHANG Hong-mei,WEN Mei,WANG Yan-qing.Study on the Interaction of 2,4,6-Trichlorophenol and Trypsin by Fluorescence Spectrometry[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2010(10). |
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| Authors: | ZHOU Qiu-hua ZHANG Hong-mei WEN Mei WANG Yan-qing |
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| Institution: | ZHOU Qiu-hua,ZHANG Hong-mei,WEN Mei,WANG Yan-qing*(Jiangsu Provincial Key Lab.of Coastal Wetland Bioresources and Environmental Protection,Research Institute of Applied Chemistry and Environmental Engineering,Yancheng Normal College,Yancheng 224002,China) |
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| Abstract: | As shown by the results of fluorescence spectrometric study on the interaction between 2,4,6-trichlorophenol and trypsin the quenching of the intrinsic fluorescence of trypsin by the presence of 2,4,6-trichlorophenol was a static quenching process due to the formation of a compound complex between the reactants through the actions of electrostatic and hydrophobic forces.The apparent binding constant K,number of binding sites and thermodynamic parameters of the reaction were calculated.Effects of 2,4,6-trich... |
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| Keywords: | Fluorescence spectrosmetry Binding reaction 2 4 6-Trichlorophenol Trypsin |
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