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Microcalorimetric Studies of the Toxic Action of La^3+ on Halobacterium Halobium R1 Growth
引用本文:刘鹏 刘义 谢之雄 陈西贵 赵儒铭 沈萍 屈松生. Microcalorimetric Studies of the Toxic Action of La^3+ on Halobacterium Halobium R1 Growth[J]. 中国化学, 2003, 21(6): 693-697. DOI: 10.1002/cjoc.20030210623
作者姓名:刘鹏 刘义 谢之雄 陈西贵 赵儒铭 沈萍 屈松生
作者单位:[1]DepartmentofChemistry,CollegeofChemistryandMolecularSciences,WuhanUniversity,Wuhan,Hubei430072,China [2]SchoolofLifeSciences,WuhanUniversity,Wuhan,Hubei430072,China
基金项目:ProjectsupportedbytheNationalNaturalScienceFoundationofChina (Nos .3 0 170 0 10and 2 9973 0 3 0 ),theNaturalScienceFoundationofHubeiProvince (No .98J0 5 2 )andtheTeachingandResearchAwardProgramforOutstandingYoungProfessorsofHighEducationIn stitutes
摘    要:
A microcalorimetric technique was used to evaluate the influence of La^3 on Halobacterium halobium R1 growth.By means of LKB-2277 bioactivity monitor,ampoule methos at 37℃,the thermogenic curves of Halobacterium halobium R1 growth were obtained.In order to analyze the results,the maximum power Pm and the growth rate constants k were determined,showing that values of Pm and k are linked to the concentration of La^3 .Addition of low concentration of La^3 can cause a decrease of the maximum heat production and growth rate constant.However,high concentration of La^3 may promote growth of Halobacterium halobium R1,but at much higher concentration of La^3 ,the growth of Halobacterium halobium R1 is inhibited again.For comparison,the shapes of Halobacterium halobium R1 cell were observed by means of transmission electron microscope.According to the thermogenic curves and TEM photos of Halobacterium halobium R1 under different conditions,it is clear that metabolic mechanism of Halobacterium halobium R1 growth is changed with the addition of La^3 .

关 键 词:微量热分析 盐杆菌 生长 毒理作用 La^3+ 镧离子 热动力学 新陈代谢

Microcalorimetric Studies of the Toxic Action of La~(3+) on Halobacterium Halobium R1 Growth
Liu Peng,Liu Yi,Chen You‐Gui,Qu Song‐Sheng,Xie Zhi‐Xiong,Zhao Ru‐Ming,Shen Ping. Microcalorimetric Studies of the Toxic Action of La~(3+) on Halobacterium Halobium R1 Growth[J]. Chinese Journal of Chemistry, 2003, 21(6): 693-697. DOI: 10.1002/cjoc.20030210623
Authors:Liu Peng  Liu Yi  Chen You‐Gui  Qu Song‐Sheng  Xie Zhi‐Xiong  Zhao Ru‐Ming  Shen Ping
Abstract:
A microcalorimetric technique was used to evaluate the influence of La3+ on Halobacterium halobium Rl growth. By means of LKB‐2277 bioactivity monitor, ampoule method at 37 °C, the thermogenic curves of Halobacterium halobium R1 growth were obtained. In order to analyze the results, the maximum power Pm and the growth rate constants k were determined, showing that values of Pm and k are linked to the concentration of La3+. Addition of low concentration of La3+ can cause a decrease of the maximum heat production and growth rate constant. However, high concentration of La3+ may promote growth of Halobacterium halobium R1, but at much higher concentration of La3+, the growth of Halobacterium halobium R1 is inhibited again. For comparison, the shapes of Halobacterium halobium R1 cell were observed by means of transmission electron microscope. According to the thermogenic curves and TEM photos of Halobacterium halobium R1 under different conditions, it is clear that metabolic mechanism of Halobacterium halobium R1 growth is changed with the addition of La3+.
Keywords:Halobacterium halobium R1   microcalorimetry   ther mokinetics   metabolism   La 3+
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