New conditions for matrix-assisted laser desorption/ionization mass spectrometry of native bacterial R-type lipopolysaccharides |
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Authors: | Sturiale Luisa Garozzo Domenico Silipo Alba Lanzetta Rosa Parrilli Michelangelo Molinaro Antonio |
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Institution: | CNR-Istituto di Chimica e Tecnologia dei Polimeri, Viale Regina Margherita 6, 95123 Catania, Italy. |
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Abstract: | A new sample preparation method for matrix-assisted laser desorption/ionization (MALDI) analysis of native rough-type lipopolysaccharides (R-type LPSs) is presented. In our MALDI mass spectra, besides the M--H](-) ions, abundant ions originating from the cleavage between the 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) unit and the lipid A moiety are always present, giving important pieces of information about the structure of the molecules analyzed. Remarkably, in most cases, the comparison of the MALDI mass spectra of the intact R-type LPS with the O-deacylated one allowed us to obtain the structure of the lipid A moiety. |
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