脱氢枞氧基聚氧乙烯缩水甘油醚接枝羟乙基壳聚糖水凝胶制备及其性能

通过脱氢枞醇聚氧乙烯（10）醚（DA（EO）₁₀H）与环氧氯丙烷缩合后，再在NaOH存在下脱氯化氢得到脱氢枞氧基聚氧乙烯（10）缩水甘油醚（DA（EO）₁₀GE），然后通过DA（EO）₁₀GE对羟乙基壳聚糖（HECTS）的接枝制备DA（EO）₁₀GE接枝羟乙基壳聚糖（DA（EO）₁₀GE-g-HECTS），最后利用Genipin对DA（EO）₁₀GE-g-HECTS进行交联，得到DA（EO）₁₀GE-g-HECTS/Genipin水凝胶。研究结果表明：DA（EO）₁₀GE对HECTS糖单元摩尔比的增加能提高DA（EO）₁₀GE-g-HECTS的接枝度，并延长其与Genipin交联形成凝胶的时间；增加Genipin的用量可提高接枝产物与Genipin交联形成凝胶的能力；负载于DA（EO）₁₀GE-g-HECTS/Genipin凝胶中的氯霉素在人工肠液中的累积释放率与时间的关系，可以很好地用Boltzmann函数描述，且提高接枝度和降低Genipin用量有利于提高药物的最终累积释放率；低接枝度DA（EO）₁₀GE-g-HECTS经Genipin交联形成的载药凝胶，其药物释放行为符合一级动力学方程的特征。

Preparation of Hydrogels Based on Dehydroabietyl Polyoxyethylene Glycidyl Ether Grafted Hydroxyethyl Chitosan and Their Properties

Dehydroabietol polyoxyethylene（10） ether （DA（EO）₁₀H） is condensated with epichlorohydrin in the presence of boron fluoride diethyl etherate firstly， and then dehydroabietyl polyoxyethylene glycidyl ether （DA（EO）₁₀GE） is obtained through dehydrochlorination of the condensation product in the presence of NaOH. DA（EO）₁₀GE grafted hydroxyethyl chitosan （DA（EO）₁₀GE-g-HECTS） is prepared through grafting DA（EO）₁₀GE to hydroxyethyl chitosan （HECTS）. The hydrogels based on DA（EO）₁₀GE-g-HECTS are prepared through cross-linking reaction between Genipin and DA（EO）₁₀GE-g-HECTSs. The increase of molar ratio of DA（EO）₁₀GE versus sugar unit could result in the increase of grafting degree （DG） of DA（EO）₁₀GE-g-HECTSs， the gelation time of DA（EO）₁₀GE-g-HECTS/Genipin with high DG is longer than that with low DG， and increasing the Genipin amount could improve the capability of DA（EO）₁₀GE-g-HECTS/Genipin to form hydrogel. The relation between the cumulative release rate of Chloramphenicol loaded in DA（EO）₁₀GE-g-HEC/GE gel and times in artificial intestinal fluid could be well described by Boltzmann function， and the final cumulative release rate could be improved by increasing the DG or decreasing the Genipin dosage. The release behavior of drug loaded in the hydrogel which is formed by the cross-linking reaction between DA（EO）₁₀GE-g-HECTS with low DG and Genipin accords with the characteristics of first-order kinetic equation.