Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis |
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| Authors: | Matteo Grattieri Sofia Babanova Carlo Santoro Edoardo Guerrini Stefano PM Trasatti Pierangela Cristiani Massimiliano Bestetti Plamen Atanassov |
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| Institution: | 1. Department of Chemistry, Materials and Chemical‐Engineering, Politecnico di Milano, Piazza Leonardo Da Vinci, 32, 20133 Milan, Italy;2. Department of Chemistry , Università degli Studi di Milano, Via Golgi 19, 20133?Milan, Italy;3. Center for Emerging Energy Technologies, Department of Chemical & Nuclear Engineering, Center for Emerging Energy Technologies, University of New Mexico, Albuquerque, NM 87131, USA;4. RSE – Ricerca sul Sistema Energetico S.p.A., Sustainable Development and Energy Sources Department, 20133 Milan, Italy |
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| Abstract: | A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single‐chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer‐controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3 mg L?1 of O2 in the bulk solution, decreasing to 0 mg L?1 in the cathodic biofilm. The MFC generated power in the range of 0–0.08 mW, associated to the oxygen content. |
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| Keywords: | Biosensor Bilirubin oxidase Oxygen measurement Biofilm Biocathode MFC |
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