Polarographic determination of α-amylase activity |
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Authors: | Galina Strand Reinhard Renneberg Frieder Scheller |
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Affiliation: | Central Institute of Molecular Biology of the Academy of Science, GDR 1115 Berlin - Buch G.D.R. |
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Abstract: | The enzyme α-amylase splits blue starch in fragments bearing electroactive groups which exhibit two waves in the pulse polarograms. This behaviour is the basis of the polagraphic determination of bacterial and human serum α-amylase activity. The differential pulse mode is more sensitive by a factor of 25 as compared with normal pulse polarography. With serum α-amylase, protein adsorption disturbs the determination of low activities. |
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Keywords: | To whom correspondence should be addressed. |
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