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Aqueous extracts of Agave sisalana boles have prebiotic potential
Authors:Alexsandra Conceição Apolinário  Antonio Diogo Silva Vieira  Susana Marta Isay Saad  Adalberto Pessoa Jr  José Alexsandro da Silva
Institution:1. Department of Biochemical and Pharmaceutical Technology, University of S?o Paulo, S?o Paulo, 05508-000, Brazil;2. Graduate Program of Pharmaceutical Sciences, State University of Paraíba, Campina Grande, Paraíba, Brazil;3. Department of Biochemical and Pharmaceutical Technology, University of S?o Paulo, S?o Paulo, 05508-000, Brazil;4. Department of Agrarian and Exact Sciences, Campus IV – UEPB, Post-Graduate Program in Agroindustry Systems, Federal University of Campina Grande, Pombal, Paraíba, Brazil
Abstract:Abstract

This work aimed at evaluating the prebiotic potential of the aqueous extract and crude polysaccharides from Agave sisalana boles by an in vitro screening. Crude polysaccharides were obtained from the aqueous bole extract by precipitation with acetone and resuspension in water. The liquid extract and the polysaccharide solution were then spray dried and submitted to thermal analysis and quantification of metabolites. Prebiotic activity was checked on probiotic strains belonging to the Lactobacillus genus using inulin, fructo-oligosaccharides, fructose and glucose as positive controls. The powder of A. sisalana bole extract, which has recently been identified as a rich source of inulin, exhibited higher potential of fermentation compared with crude polysaccharides.
Keywords:Agave sisalana  aqueous extract  crude polysaccharides  prebiotic activity  Lactobacillus sp
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