首页 | 本学科首页   官方微博 | 高级检索  
     


Optimization of polysaccharides extraction from quince peels: partial characterization,antioxidant and antiproliferative properties
Authors:Mariem Itaimi Dammak  Zeineb Mzoughi  Ibtissem Chakroun  Hedi Ben Mansour  Didier Le Cerf
Affiliation:1. Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Science de Monastir, Université de Monastir, Monastir, Tunisia;2. Unité de Recherche Analyses et Procédés Appliqués à l’Environnement UR17ES32-Institut Supérieur des Sciences Appliquées et de Technologie de Mahdia, Université de Monastir, Monastir, Tunisia;3. Normendie Université, Laboratoire de Polymères Biopolymères Surfaces (PBS), UMR 6270 &4. FR3038CNRS, Université de Rouen, Mont Saint Aignan, France
Abstract:Abstract

In this study, Box-Behnken Design was used to optimize the ultrasonic extraction of polysaccharides from quince peels (QPPs) by ascorbic acid and the effect of extraction temperature, extraction time and pH was evaluated. Under optimized conditions of temperature 90?°C, 60?min sonication time and pH?=?3.26, the extraction yield, the galacturonic acid yield and the concentration of sample required to scavenge 50% of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) values of QPPs were respectively 10.25%, 3.86% and 1.35?mg/mL. The QPPs extracted under optimum conditions was characterized by Fourier transform infrared spectroscopy (FTIR), Nuclear magnetic resonance (1?H NMR) and Size exclusion chromatography (SEC/MALS/VD/DRI). The monosaccharide analysis revealed that arabinose was the most abundant, followed by galactose, glucose, mannose and xylose. Moreover, QPPs showed significant antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric- reducing antioxidant power (FRAP)) and reduced viability of human Caco-2 and murine B-16 cell lines in a dose-dependent manner. Hence QPPs could be used as antitumor agent in functional foods andpharmaceutical industries.
Keywords:Quince  polysaccharides  ascorbic acid  ultrasound-assisted extraction  physico-chemical characterization  antioxidant and antiproliferative activities
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号