Metabolite profiling and in-vitro colon cancer protective activity of Cycas revoluta cone extract |
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| Authors: | Samit Bera Bhaskar Das Arnab De Atish Barua Susmita Das Bratati De |
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| Institution: | 1. Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India;2. Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, Kolkata, India;3. Phytochemistry and Pharmacognosy Laboratory, Department of Botany, University of Calcutta, Kolkata, India |
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| Abstract: | AbstractThe methanolic extract of Cycas revoluta cone (MECR) was analyzed by GC-MS and UHPLC for metabolite profiling and was evaluated for anti-colon cancer property by using in vitro assays like Cell Viability Assay, Colony Formation Assay, ROS Determination, Flowcytometry, DAPI staining assay, Tunel assay. GC-MS and HPLC analysis confirmed the presence of different phytochemicals in the extract of Cycas revoluta cone. In-vitro studies showed MECR extract showed significant anti-colon cancer activity by reducing proliferation and inducing apoptosis in colon cancer cell (HCT-8) line, but no such activity was seen in normal colon cell (CCD-18Co) line. The investigation confirms that MECR may be a promising candidate in colon cancer protection. |
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| Keywords: | Cycas revoluta colon cancer reactive oxygen species (ROS) GC-MS |
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