| Total Synthesis and Expressions in E. coli of Three Redesigned Genes for the Mature Small Subunit (rbcS) of Rubisco Fron Tobacco, Wheat and Rice |
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| 作者姓名： | CHEN Hai-Bao Shanghai Institute of Organic Chemistry Academia Sinica Shanghai |
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| 作者单位： | CHEN Hai-Bao Shanghai Institute of Organic Chemistry，Academia Sinica，200032 Shanghai |
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| 基金项目： | These studies were supported by grants from the High Technology Development Program of China, from the National Lab of Bioorganic and Natural Products Chemistry and from the Lab of Computer Chemistry, Chinese Acaedemy of Sciences |
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| 摘 要： | Three structural genes, which code for the mature small subunits (rbcS) of ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco, wheat and rice, respectively,have been redesignedwith the aid of a computer,totally synthesized by combining chemical method and enzymatic ligation,and expressed in E. coli with high yields.
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Total Synthesis and Expressions in E. coli of Three Redesigned Genes for the Mature Small Subunit (rbcS) of Rubisco Fron Tobacco, Wheat and Rice |
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| CHEN Hai-Bao Shanghai Institute of Organic Chemistry,Academia Sinica, Shanghai.Total Synthesis and Expressions in E. coli of Three Redesigned Genes for the Mature Small Subunit (rbcS) of Rubisco Fron Tobacco, Wheat and Rice[J].Chinese Journal of Organic Chemistry,1993(3). |
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| Authors: | CHEN Hai-Bao |
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| Institution: | CHEN Hai-Bao Shanghai Institute of Organic Chemistry,Academia Sinica,200032 Shanghai |
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| Abstract: | Three structural genes, which code for the mature small subunits (rbcS) of ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco, wheat and rice, respectively,have been redesignedwith the aid of a computer,totally synthesized by combining chemical method and enzymatic ligation,and expressed in E. coli with high yields. |
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| Keywords: | gene design synthetic gene gene expression RubisCO rbcS |
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