亲水作用色谱及其在环境分析中的应用进展

亲水作用色谱(HILIC)是一种分离极性和亲水性化合物的液相色谱模式,其作为反相液相色谱(RPLC)的重要补充,近年来越来越受到各个领域的关注和重视。这不只是因为强极性化合物的分离问题在各个领域引起了重视,而且因为与RPLC比较,HILIC具有流动相组成黏度低、色谱柱渗透性好、与质谱联用的灵敏度高及反压较低等优势。本文简要概述了HILIC的发展历程、特点及保留机理,重点介绍了HILIC用于环境分析的最新进展,评述了HILIC及RPLC用于污染物分析的优缺点,并指出了HILIC用于环境分析的未来发展趋势。

Hydrophilic interaction chromatography and its recent applications in environmental analysis

Hydrophilic interaction chromatography (HILIC) has been used as a supplement method of reversed-phase liquid chromatography (RPLC) for the separation of the polar and hydrophilic compounds, and has been received more and more attention in recent years. One reason is that the separation problems of strongly polar compounds are rising in various fields. Another is that there are some obvious advantages of HILIC compared with RPLC in the separation of polar compounds, such as low viscosity of mobile phase, good permeability of the column, high sensitivity when coupled to mass spectrometry and low back pressure. In this review, the development history, characteristics and retention mechanism of HILIC are summarized. The main attention in this review is on the applications of HILIC in environmental analysis. In addition, the advantages and disadvantages of HILIC and RPLC in the separation of pollutants are discussed, and the development trends of HILIC applications in environmental analysis are proposed.