| 硅胶聚合物键合相键合基团及流动相盐浓度和pH值对蛋白质分离的影响 |
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| 引用本文： | 魏芸,樊立民.硅胶聚合物键合相键合基团及流动相盐浓度和pH值对蛋白质分离的影响[J].分析化学,1998,26(10):1218-1221. |
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| 作者姓名： | 魏芸 樊立民 |
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| 作者单位： | 中国科学院兰州化学物理研究所!兰州730000 |
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| 摘 要： | 介绍了３种同孔径不同键合基团的大孔径硅胶聚合物键上的合成，３种填料是在１００ｎｍ孔径硅胶上键合乙烯基后，分别与甲基丙烯酸羟乙基酯，二乙烯基苯共聚形成ＨＤＳＩ填料；与甲基丙烯酰胺共聚形成ＰＡＭＳＩ填料；与顺丁烯二酸共聚再与乙二胺反应形成ＰＦＭＳＩ填料。比较了它们对蛋白质分离的特性。
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| 关 键 词： | 硅胶聚合 键合相 蛋白质 分离 HPLC 固定相 |
Preparation of Silicage-Polymer - Bonded Phase and the Effect of Different Agent, pH and Salt Concentration in Elution on Proteins Separation |
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| Wei Yun,Fan Limin,Zhang Junyan,Jiang Shengxiang,Chen Liren.Preparation of Silicage-Polymer - Bonded Phase and the Effect of Different Agent, pH and Salt Concentration in Elution on Proteins Separation[J].Chinese Journal of Analytical Chemistry,1998,26(10):1218-1221. |
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| Authors: | Wei Yun Fan Limin Zhang Junyan Jiang Shengxiang Chen Liren |
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| Abstract: | The synthesis of three (100 nm) macroporous silicage-polymer- bonded packings (HDSI, PAMSI, PFMSI) and their chromatographic characteristics in the separation of proteins are introduced. The results show that macroporous packings all can separate proteins quickly. The effect of salt concentration in elution on the retention of proteins on PAMSI packing is discussed. The effect of pH in mobile phase on the retention of proteins on PFMSI packing is also discussed. |
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| Keywords: | Silicage - polymer- bonded phase protein separation |
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