气相质谱法对小鼠体内甲苯二异氰酸酯代谢产物的鉴定分析

应用气相质谱测定小鼠体内甲苯二异氰酸酯(TDI)的代谢产物，并鉴别了其在机体中的代谢途径。TDI色谱条件为Supelco PTETM-5色谱柱(30 mm×0.25 mm×0.25 μm)，起始柱温90 ℃保持30 min，以40 ℃·min-1升至280 ℃，保持5.25 min；汽化室温度250 ℃；载气为氦气，流速为1.0μL·min-1。TDI体内代谢产物色谱条件为30+2mX0.25+0.02mm 94%甲基、4%乙烯基键合相弹性石英毛细柱，起始温度30 ℃保持5 min，以8 ℃·min-1升至280 ℃，保持5 min。汽化室温度250 ℃；载气为氦气，流速1.0μL·min-1。质谱条件为电离方式EI，电离能量70eV，连接管温度280 ℃，扫描范围35～350μ；进样量1.0μL。结果表明2，4甲苯二异氰酸酯在血液、尿液、粪便中都被代谢成为2，4甲苯二胺。GC-MS法可有效分离并鉴定TDI体内代谢产物。

Determination and Analysis of Toluene Diisocyanate Metabolites in Mice Using Gas Chromatography-Mass Spectrometry

In the present research we used gas chromatography-mass spectrometry (G C-MS) to determine metabolites of toluene diisocyanate (TDI) in mice and deduce the pathway for toluene diisocyanate metabolism in the organism. Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column (30 mm x 0.25 mm x 0.25 microm); initial column temperature: 90 degrees C, which was maintained for 30 min, then the temperature was increased at a rate of 40 degrees C x min(-1) to 280 degrees C, and maintained for 5.25 min; temperature for the vaporizing chamber: 250 degrees C; carrier gas: helium flowing at 1.0 microL x min(-1). Conditions for chromatography of TDI metabolites in the organism: 94% methyl, 4% ethenyl-bonded-phase fused-silica capillary column (30 + 2 m x 0.25 + 0.02 mm); initial column temperature: 30 degrees C, which was maintained for 5 min, after and then was increased at a rate of 80 degrees C x min(-1) to 280 degrees C, and maintained for 5 min; temperature for the vaporizing chamber: 250 degrees C; carrier gas: helium flowing at 1.0 microL x min(-1). Conditions for mass spectrometry: EI for ionization; 70 eV for ionization energy; 280 degrees C for connecting tube temperature; 35-350 micro for range of scanning; and 1.0 microL for sample size. The results showed that 2 ,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene. Under the conditions selected for GC-MS, TDI metabolites in the organism can be isolated and identified.