Alkali-stable high-pI isoelectric membranes for isoelectric trapping separations |
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Authors: | Lalwani Sanjiv Shave Evan Fleisher Helen C Nzeadibe Kingsley Busby M Brent Vigh Gyula |
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Institution: | Department of Chemistry, Texas A&M University, College Station, TX 778452-3012, USA. |
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Abstract: | Alkali-stable, high-pI isoelectric membranes have been synthesized from quaternary ammonium derivatives of cyclodextrins and poly(vinyl alcohol), and bifunctional cross-linkers, such as glycerol-1,3-diglycidyl ether. The new, high-pI isoelectric membranes were successfully applied as cathodic membranes in isoelectric trapping separations in place of the hydrolytically more labile, polyacrylamide-based cathodic isoelectric membranes, and permitted the use of catholytes as alkaline as 1 M NaOH. The new high-pI isoelectric membranes have shown excellent mechanical stability, low electric resistance and long life times, even when subjected to electrophoresis with current densities as high as 80 mA/cm2. |
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