高效液相色谱法制备人胆汁中结合胆红素

针对单结合胆红素（ｍｏｎｏｃｏｎｊｕｇａｔｅｄｂｉｌｉｒｕｂｉｎ，ＭＣＢ）理化性质极不稳定而造成对其分析、制备的困难性，在Ｓｐｉｖａｋ和Ｔｒｏｔｍａｎ方法的基础上建立了一个简单可行的人胆汁结合胆红素（ｃｏｎｊｕｇａｔｅｄｂｉｌｉｒｕｂｉｎ，ＣＢ）高效液相色谱（ＨＰＬＣ）二次洗脱的制备方法，并对方法的可靠性作了论证，得到了ＭＣＢ较为满意的量与纯度，为进一步探讨ＭＣＢ的理化性质及其与胆石形成的关系提供了条件。

The Preparation of the Conjugated Bilirubins in the Human Bile by High Performance Liquid Chromatography

Cholelithiasis is a prevalant disease of human being with a rather complicated mechanism of its pathogenesis. Recently an increasing date available suggest that monoconjugated bilirubin (MCB) in the bile may play an important role in the formation of the gallstones However,little knowledge has been gained about the physical chemical properties and preparation of MCB because of its little stability.In order to explore the physical chemistry of MCB and its critical role in the formation of the gallstones and obtain a larger amount of pure MCB ,a modified preparative procedure of Spivak's and Trotman' s by high performance liquid chromatography has been developed, named duplex elution preparation method,with the following advantages:(1) A more satisfactory elution system in preparation procedure;(2) A quite good stability in a long time with the methanol solution of MCB;(3) A more complete protein precipitation with methanol dilution and freeze of bile which can be preserved conveniently;(4) More rapid and simplified preparation processes which meet the requirement of the physical chemistry. study and explore the important role in the formation of the gallstones of the MCB conveniently;(5) A rather high purity of diconjugated bilirubin (DCB) (89%) and MCB (85%) produced by this method.