| 分光光度法测定黄瓜籽粉中盐酸小檗碱的含量 |
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| 引用本文： | 张程,宋雪慧,于博,包玉敏,张力.分光光度法测定黄瓜籽粉中盐酸小檗碱的含量[J].光谱实验室,2010,27(3):1103-1105. |
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| 作者姓名： | 张程 宋雪慧 于博 包玉敏 张力 |
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| 作者单位： | 1. 内蒙古通辽市产品质量计量检测所,内蒙古通辽市科尔沁区霍林河大街479号,028000
2. 内蒙古民族大学化学化工学院,内蒙古通辽市霍林河大街22号,028043 |
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| 摘 要： | 采用超声波法在2种条件下即(1)先超声处理,后放置过夜;(2)先放置过夜,后超声处理,对黄瓜籽粉中盐酸小檗碱进行提取,利用分光光度法测定盐酸小檗碱的含量,检测波长为426nm。回归方程y=0.00393+1.28929x(mg/10mL),r=0.9999;盐酸小檗碱浓度在0.099—0.796mg/10mL范围内与吸光度呈线性关系。2种方法的平均含量分别为2.4043、1.4797mg/g。平均回收率分别为94.96%、107.32%。实验结果表明,方法(1)较方法(2)提取率高。分光光度法用于黄瓜籽粉中盐酸小檗碱的含量测定,简便,可靠,快捷。
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| 关 键 词： | 盐酸小檗碱 黄瓜籽粉 分光光度法 |
Determination of Berberine Hydrochloride in Cucumber Seed Powder by Spectrophotometry |
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| ZHANG Cheng,SONG Xue-Hui,YU Bo,BAO Yu-Min,ZHANG Li.Determination of Berberine Hydrochloride in Cucumber Seed Powder by Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2010,27(3):1103-1105. |
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| Authors: | ZHANG Cheng SONG Xue-Hui YU Bo BAO Yu-Min ZHANG Li |
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| Institution: | a(Tongliao Institute of Product Quality ＆ Measure Inspection,Tongliao,Inner Mongolia 028000,P.R.China)a(College of Chemistry and Chemical Engineering,Innre Mongolia Uniuersity for Nationalities,Tongliao,Inner Mongolia 028043,P.R.China) |
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| Abstract: | Berberine hydrochloride in the cucumber seed powder was extracted by two methods：（1） firstly ultrasound,and then static overnight;（2） firstly static overnight,then ultrasound.The contents of berberine hydrochloride was determined by spectrophotometry at 426nm.The concentration of berberine hydrochloride has good liner relationship with absorbency in the range of 0.099—0.796mg/10mL,and regression equation is y=0.00393＋1.28929x（mg/10mL）,r=0.9999.The average contents are 2.4043mg/g and 1.4797mg/g for two methods,respectively.The average recoveries are method（1）：94.96%,method（2）：107.32%.Methods（1） is better than methods（2） in extracting berberine hydrochloride.Spectrophotometry is simple,rapid and suitable for the determination of the contents of berberine hydrochloride. |
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| Keywords: | Berberine Hydrochloride Cucumber Seed Powder Spectrophotometry |
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