| 胍基修饰硅介孔材料用于蛋白质酸性条件酶解 |
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| 引用本文： | 孙灏,张莹,陆豪杰,杨芃原.胍基修饰硅介孔材料用于蛋白质酸性条件酶解[J].中国科学:化学,2011(4):756-761. |
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| 作者姓名： | 孙灏 张莹 陆豪杰 杨芃原 |
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| 作者单位： | 复旦大学化学系和生物医学研究院; |
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| 基金项目： | 上海高校特聘教授(东方学者)岗位计划; 上海市教育委员会和上海市教育发展基金会“曙光计划”; 上海市重点学科(B109); 国家自然科学基金(21025519和21005020)资助 |
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| 摘 要： | 在介孔材料FDU-12上用后修饰法成功合成了带有强碱性基团胍基的全新的功能化硅介孔材料Gua-FDU-12.利用该新型硅介孔材料成功实现了在酸性溶液中进行蛋白质酶解,并成功应用于反相色谱分离的蛋白质的直接酶解.该方法解决了传统的基于trypsin酶解无法在酸性条件下工作的难题,因而省去了top-down策略中反相色谱分离与蛋白酶解之间需要加入大量溶液来调节pH的步骤,为将该方法进一步应用于基于top-down策略的蛋白质组研究奠定了基础.
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| 关 键 词： | 介孔材料 酶解 top-down 质谱 |
Application of guanidine functionalized mesoporous silicate for protein proteolysis in acid solution |
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| SUN Hao,ZHANG Ying,LU HaoJie & YANG PengYuan.Application of guanidine functionalized mesoporous silicate for protein proteolysis in acid solution[J].Scientia Sinica Chimica,2011(4):756-761. |
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| Authors: | SUN Hao ZHANG Ying LU HaoJie & YANG PengYuan |
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| Institution: | SUN Hao,ZHANG Ying,LU HaoJie & YANG PengYuan Department of Chemistry and Institutes of Biomedical Sciences,Fudan University,Shanghai 200433,China |
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| Abstract: | A novel functionalized mesoporous silicate Gua-FDU-12,which introduces a strong alkaline functional group-guanidine on FDU-12,has been successfully synthesized by post-synthesis method.With this material,proteins are well digested in acid environment.Also,it has been successfully applied to direct proteolysis of proteins separated from RPLC.This method has solved the problem that trypsin cannot work in acid environment.Therefore,in atop-downstrategy,proteins eluted from RPLC can be directly digested instead... |
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| Keywords: | mesoporous silicates proteolysis top-down mass spectrometry |
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