三维荧光光谱结合二阶校正方法测定细胞培养基中阿霉素的含量

本文采用交替三线性分解(ATLD)和交替归一加权残差三线性分解(ANWE)两种二阶校正方法结合激发发射矩阵荧光光谱对完全不经任何预处理的细胞培养基中的阿霉素进行简单、快速、直接的定量测定.当算法选取组分数为2时,解析得到细胞培养基中阿霉素的平均回收率分别为(100.5±1.8)%和(100.3±1.9)%.在细胞培养基中加入烟酰胺腺嘌呤二核苷酸(NADH)、烟酰胺腺嘌呤二核苷酸磷酸(NADPH)、黄素腺嘌呤二核苷酸(FAD)和黄素单核苷酸(FMN)四种细胞内的自发荧光物后,选取组分数为4时,解析得到细胞培养基中阿霉素的平均回收率分别为(99.1±2.9)%和(99.2±3.1)%.结果表明该分析方法能够准确、快速地直接测定细胞培养基中阿霉素的含量,并且在模拟细胞内荧光干扰环境下可定量测定阿霉素,且能获得令人满意的结果.

Determination of adriamycin in cell culture media by using excitation-emission matrix fluorescent spectra coupled with second-order calibration

This paper reports a simple and rapid method for quantitative analysis of adriamycin in cell culture media without any pretreatment procedure. It combined second-order calibration methods based on the alternating trilinear decomposition （ATLD） and the alternating normalization-weighter error （ANWE） algorithms, respectively, with excitation-emission matrix fluorescent spectra. When the component number in the case was set to 2, the average recoveries of adriamycin in cell culture media were （100.5±1.8）% and （100.3±1.9）%, respectively. The satisfactory results showed that the second-order calibration method can be applied to directly quantify adriamycin in cell culture media. Moreover, in the analysis of cell culture media including four interferences which are the fluorescent materials in cell, the average recoveries attained from ATLD and ANWE with the factor number of 4 were （99.1±2.9）% and （99.2±3.1）%, respectively. It was found that second-order calibration method combined with excitation-emission matrix fluorescent spectra is appropriate for quantitative analysis of drug contents in cell culture media even in the presence of natural fluorescent interferences of cell.