The Correlations Between TCA-Glyoxalate Metabolite and Antibiotic Production of <Emphasis Type="Italic">Streptomyces</Emphasis> sp. M4018 Grown in Glycerol,Glucose, and Starch Mediums |
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Authors: | Leman Tarhan Hulya Ayar Kayalı Anıl Sazak Nevzat Şahin |
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Institution: | (1) Science Faculty, Department of Chemistry, Dokuz Eylul University, 35150 Buca, Izmir, Turkey;(2) Science and Arts Faculty, Department of Biology, Ondokuz Mayıs University, 55139 Kurupelit, Samsun, Turkey |
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Abstract: | The alterations of organic acids citrate, α-ketoglutarate, succinate, fumarate, malate production together with isocitrate
lyase activity as a glyoxalate shunt enzyme, and antibiotic production of Streptomyces sp M4018 were investigated in relation to changes in the glucose, glycerol and starch concentrations (5–20 g/L) after identification
as a strain of Streptomyces hiroshimensis based on phenotypic and genotypic characteristics. The highest intracellular citrate and α-ketoglutarate levels in 20 g/l
of glucose, glycerol, and starch mediums were 399.47 ± 4.78, 426.93 ± 6.40, 355.84 ± 5.38 ppm and 444.81 ± 5.12, 192.96 ± 2.26,
115.20 ± 2.87 ppm, respectively. The highest succinate, malate, and fumarate levels were also determined in 20 g/l of glucose
medium as 548.9 ± 11.21, 596.15 ± 8.26, and 406.42 ± 6.59 ppm and the levels were significantly higher than the levels in
glycerol and starch. Extracellular organic acid levels measured also showed significant correlation with carbon source concentrations
by showing negative correlation with pH levels of the growth medium. The antibiotic production of Streptomyces sp. M4018 was also higher in glucose medium as was the case also for organic acids when compared with glycerol. On the other
hand, there is no production in starch. |
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