| 胆固醇修饰富勒烯/γ-环糊精包结复合物的生物活性 |
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| 引用本文： | 高云燕,刘丽华,欧植泽,李嫕,杨国强,王雪松.胆固醇修饰富勒烯/γ-环糊精包结复合物的生物活性[J].物理化学学报,2010,26(2):495-501. |
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| 作者姓名： | 高云燕 刘丽华 欧植泽 李嫕 杨国强 王雪松 |
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| 作者单位： | Department of Applied Chemistry, School of Science, Northwestern Polytechnical University, Xi'an 710072, P. R. China; Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China; Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China |
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| 摘 要： | 采用Bingel-Hirsch反应合成了胆固醇修饰的富勒烯(CHL-C60), 通过核磁共振(NMR)、质谱(MS)、元素分析对CHL-C60的化学结构进行了表征. γ-环糊精(γ-CD)对甾环具有较强的包结能力, 能够与CHL-C60形成包结复合物(CHL-C60/γ-CD), 从而有效提高CHL-C60的水溶性. 紫外-可见吸收光谱和荧光光谱研究结果表明, CHL-C60能够从γ-CD的疏水空腔中解离出来, 与人血清白蛋白(HSA)及牛血清白蛋白(BSA)形成稳定的复合体, 其结合常数分别为5.73×104和7.05×104 L·moL-1. 无氧条件下, CHL-C60/γ-CD通过光诱导电子转移作用断裂pBR322质粒脱氧核糖核酸(DNA), 其效率可达60.5%.
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| 关 键 词： | 富勒烯 环糊精包结物 血清白蛋白 DNA断裂 |
| 收稿时间： | 2009-08-28 |
| 修稿时间： | 2009-11-12 |
Biological Activity of a Cholesterol Modified Fullerene/γ-Cyclodextrin Inclusion Complex |
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| GAO Yun-Yan,LIU Li-Hua,OU Zhi-Ze,LI Yi,YANG Guo-Qiang,WANG Xue-Song.Biological Activity of a Cholesterol Modified Fullerene/γ-Cyclodextrin Inclusion Complex[J].Acta Physico-Chimica Sinica,2010,26(2):495-501. |
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| Authors: | GAO Yun-Yan LIU Li-Hua OU Zhi-Ze LI Yi YANG Guo-Qiang WANG Xue-Song |
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| Institution: | Department of Applied Chemistry, School of Science, Northwestern Polytechnical University, Xi'an 710072, P. R. China; Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China; Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China |
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| Abstract: | A cholesterol modified fullerene (CHL-C60) was synthesized using the Bingel-Hirsch reaction and was characterized by nuclear magnetic resonance (NMR), mass spectra (MS), elemental analysis (EA). Results of UV-Vis spectrumand fluorescence spectrumindicate that CHL-C60 forms an inclusion complex with γ-cyclodextrin (γ-CD) due to the strong interaction between γ-cyclodextrin and the steroid ring. The water solubility of CHL-C60 is enhanced significantly. CHL-C60 can dissociate from cyclodextrin and subsequently bind to human serum albumin (HSA) and bovine serum albumin (BSA) with association constants of 5.73×104 and 7.05×104 L·moL-1, respectively. The efficiency of desoxyribonucleic acid (DNA) photocleavage for CHL-C60/γ-CD is as high as 60.5% under anaerobic conditions, which is caused by photoinduced electron transfer frompBR322 plasmid DNA to CHL-C60. |
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| Keywords: | Fullerene Cyclodextrin inclusion complex Serumalbumin DNA cleavage |
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