Stabilization of bilayer lipid membranes on solid supports by trehalose |
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| Institution: | 1. Department of Biophysics and Chemical Physics, Faculty of Mathematics and Physics, Comenius University, 84215 Bratislava, Slovakia;2. Membrane Biophysics Laboratory (Giltner Hall), Physiology Department, Michigan State University, East Lansing, MI 48824, USA;3. Center for Interface Sciences, Microelectronic Department, Slovak Technical University, 81219 Bratishlava, Slovakia;1. Institute of Computational Modelling SB RAS, Federal Research Center KSC SB RAS, Akademgorodok 50-44, 660036 Krasnoyarsk, Russia;2. Siberian Federal University, Svobodny 79, 660041 Krasnoyarsk, Russia;1. National Engineering Research Center for Rare Earth Materials, General Research Institute for Non-Ferrous Metals, Beijing 100088, China;2. Grirem Advanced Materials Co., Ltd., Beijing 100088, China;1. Department of Chemistry, Chung Yuan Christian University, Chung-Li District, Taoyuan City 32023, Taiwan, ROC;2. Research Center for Analysis and Identification, Chung Yuan Christian University, Chung-Li District, Taoyuan City 32023, Taiwan, ROC;1. Department of Liberal Studies, National Technical Institute for the Deaf, Rochester Institute of Technology, United States;2. Department of Cognitive Science, University of California, Irvine, United States;3. Department of Psychology, University of California, San Diego, United States;1. Key Laboratory of Advanced Materials (MOE), Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;2. School of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China |
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| Abstract: | Using the electrostriction method the effect of the glucose and trehalose on the elasticity modulus perpendicular to the membrane plane, E⊥, and the electrical capacitance, C, of supported bilayer lipid membranes (s-BLM) formed on the freshly cut tip of Teflon-coated Ag wire was studied. Addition of saccharides into the electrolyte resulted in a decrease in the elasticity modulus of the s-BLM formed from the soybean phosphatidylcholine in n-hexadecane, while the capacitance was increased. In addition, the trehalose has a considerable stabilizing effect on the above parameters of the s-BLM. Treatment of the s-BLM in an electrolyte containing 0.3 M of the trehalose allowed storage of the s-BLM under dry conditions and under refrigeration, with the subsequent recovery of membrane parameters after the wire had been dipped into the electrolyte. |
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