| 核壳型Fe_3O_4/Au粒子的制备及其在表面等离子体子共振传感器中的应用 |
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| 引用本文： | 王健,王丽英,孙颖,朱晓楠,徐昊妍,毕宁,张寒琦,曹彦波,王兴华,宋大千.核壳型Fe_3O_4/Au粒子的制备及其在表面等离子体子共振传感器中的应用[J].化学学报,2010,68(3):263-268. |
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| 作者姓名： | 王健 王丽英 孙颖 朱晓楠 徐昊妍 毕宁 张寒琦 曹彦波 王兴华 宋大千 |
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| 作者单位： | 吉林大学化学学院,长春,130012 |
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| 基金项目： | 国家自然科学基金(No20727003)资助项目 |
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| 摘 要： | 合成了核壳型Fe3O4/Au复合粒子,并对其形貌、光学性质进行了表征.通过外加磁场将Fe3O4/Au复合粒子与兔抗人IgG的偶联体固定于表面等离子体子共振(SPR)传感器的金基底膜上,形成了Fe3O4/Au/抗IgG敏感膜.与传统的通过巯基丙酸连接蛋白的方式相比,磁场作用固定的Fe3O4/Au/抗IgG敏感膜制备简单,易洗脱,具有良好的再生性,且在一定程度上提高了传感器的灵敏度.并对人IgG进行了测定,结果表明,传感器对于浓度范围在1.25～20.00μg·mL-1的人IgG有良好的信号响应.
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| 关 键 词： | Fe3O4/Au复合粒子 表面等离子体子共振SPR 人IgG |
| 收稿时间： | 2009-04-08 |
| 修稿时间： | 2009-09-10 |
Preparation of Core/Shell Fe_3O_4/Au Nanocomposite and Its Application to Surface Plasmon Resonance Biosensor |
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| Wang Jian,Wang Liying,Sun Ying,Zhu Xiaonan,Xu Haoyan,Bi Ning,Zhang Hanqi,Cao Yanbo,Wang Xinghua,Song Daqian.Preparation of Core/Shell Fe_3O_4/Au Nanocomposite and Its Application to Surface Plasmon Resonance Biosensor[J].Acta Chimica Sinica,2010,68(3):263-268. |
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| Authors: | Wang Jian Wang Liying Sun Ying Zhu Xiaonan Xu Haoyan Bi Ning Zhang Hanqi Cao Yanbo Wang Xinghua Song Daqian |
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| Institution: | College of Chemistry;Jilin University;Changchun 130012 |
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| Abstract: | The synthesis and characterization of core/shell Fe3O4/Au nanocomposite were performed.Furthermore,its application to a surface plasmon resonance(SPR) biosensor was mainly studied.The Fe3O4/Au nanocomposite coupled with rabbit anti-human IgG(anti-IgG) was trapped on the Au film by magnetic force and the Fe3O4/Au/anti-IgG sensing membrane was prepared.There is not a covalent link between the membrane and Au film,which simplifies the immobilization of the antibody and enhances the sensitivity of biosensor.The... |
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| Keywords: | Fe3O4/Au nanocomposite surface plasmon resonance (SPR) human IgG |
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