| 两种Taqman探针的制备及荧光检测性能比较 |
| |
| 引用本文： | 李红霞. 两种Taqman探针的制备及荧光检测性能比较[J]. 化学研究与应用, 2011, 23(8): 1057-1061 |
| |
| 作者姓名： | 李红霞 |
| |
| 作者单位： | 滨州职业学院轻纺化工学院,山东 滨州,256603 |
| |
| 摘 要： | 采用固相合成法,分别合成FAM/TAMRA和FAM/BHQ2双荧光标记的Taqman探针,得到的粗产物分别采用乙醇沉淀法、离子对反相色谱法、凝胶过滤柱脱盐法纯化.采用实时荧光PCR法,分别从感染的南瓜果实中检测到黄瓜绿斑驳花叶病毒(CGMMV),扩增曲线表明采用该实验条件制备的探针检测性能良好.在相同荧光PCR反应体系...
|
| 关 键 词： | Taqman探针 制备 检测 |
Preparation and comparison on function detecting of the two types of Taqman probes |
| |
| LI Hong-xia. Preparation and comparison on function detecting of the two types of Taqman probes[J]. Chemical Research and Application, 2011, 23(8): 1057-1061 |
| |
| Authors: | LI Hong-xia |
| |
| Affiliation: | LI Hong-xia(Department of Textile and Chemical Engineering,Binzhou Vocational College,Binzhou 256603,China) |
| |
| Abstract: | By adopting solid-phase synthesis,synthesize Taqman probes with double fluorescence-labeled FAM/TAMRA and FAM/BHQ2 respectively.The obtained crude products purified by applying methods of ethanol precipitation,reversed-phase ion-pair chromatography and gel-filtration desalting.By real time fluorescence PCR,the amplified curves showed that the probes work well by comparing the results of respectively detected Cucumber Green Mottle Mosaic Virus(CGMMV)from infected pumpkin fruit.Under the same fluorescent PCR ... |
| |
| Keywords: | Taqman probe preparation detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
