| 丝瓜种子萌芽及子叶生长过程中谷氨酰胺合成酶同工酶的变化 |
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| 引用本文： | 张吉,王云华,袁永泽,周卫,陆彬彬,张楚富.丝瓜种子萌芽及子叶生长过程中谷氨酰胺合成酶同工酶的变化[J].武汉大学学报(理学版),2004,50(4):511-514. |
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| 作者姓名： | 张吉 王云华 袁永泽 周卫 陆彬彬 张楚富 |
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| 作者单位： | 武汉大学,生命科学学院/发育生物学教育部重点实验室,湖北,武汉,430072 |
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| 基金项目： | 国家自然科学基金资助项目(30270130) |
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| 摘 要： | 测定了丝瓜种子萌芽及子叶不同生长阶段的谷氨酰胺合成酶(GS)的活性及其同工酶的变化．除了发育上的变化外，光和外源氮对GS活性及其同工酶影响也是显著的．无论外源氮(N)是否存在，GS活性在种子萌芽和子叶生长初期是逐渐升高的，于第6天达到最大，其后活性逐步降低．在光照下，外源氮对GS1诱导作用大于GS2；在无氮下，光对GS2的刺激大于GS1；在暗处，外源氮对GS1和GS2两者都有诱导作用．
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| 关 键 词： | 谷氨酰胺合成酶 同工酶 萌发 子叶 丝瓜 |
| 文章编号： | 1671-8836(2004)04-0511-04 |
| 修稿时间： | 2004年5月13日 |
Changes of Glutamine Synthetase Isozymes During Seed Germination and Cotyledon Development of Towel Gourd |
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| ZHANG Ji,WANG Yun-hua,YUAN Yong-ze,ZHOU Wei,LU Bin-bin,ZHANG Chu\|fu try of Education for Developmental Biology,Wuhan ,Hubei,China.Changes of Glutamine Synthetase Isozymes During Seed Germination and Cotyledon Development of Towel Gourd[J].JOurnal of Wuhan University:Natural Science Edition,2004,50(4):511-514. |
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| Authors: | ZHANG Ji WANG Yun-hua YUAN Yong-ze ZHOU Wei LU Bin-bin ZHANG Chu\|fu try of Education for Developmental Biology Wuhan Hubei China |
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| Institution: | ZHANG Ji,WANG Yun-hua,YUAN Yong-ze,ZHOU Wei,LU Bin-bin,ZHANG Chu\|fu try of Education for Developmental Biology,Wuhan 430072,Hubei,China) |
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| Abstract: | The changes of glutamine synthetase (GS) and its isozymes were determined during seed germination and cotyledon developmental of towel gourd. The activities of GS and its isozymes were influenced by light and external nitrogen besides the development effect. Whether external nitrogen exist or not, GS activity was increased gradually in the seed germination and initial stage of cotyledon development and was the highest in 6 d. After this, the activity was declined. Under light, the induced activity of GS1 was higher than that of GS2. In external nitrogen-free, the promoted GS2 activity was more than that of GS1. Under the dark, the activities of both GS1 and GS2 were increased by the external nitrogen. |
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| Keywords: | glutamine synthetase isozyme germination cotyledon towel gourd |
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