| improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip |
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| 引用本文： | Li Hua CHEN. improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip[J]. 中国化学快报, 2006, 17(12): 1619-1622 |
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| 作者姓名： | Li Hua CHEN |
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| 作者单位： | Li Hua CHEN? College of chemistry and molecular engineer，Qingdao University of Science and Technology，Qingdao 266042 |
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | Recently, surface plasmon resonance (SPR) become more and more popular without the need of the label technology1-3. However, sometimes, a number of experimental artifacts complicate the final biosensor analysis4-7. The utilization of a reference surface c…
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| 关 键 词： | 牛血清白蛋白 BSA修饰芯片 谷胱甘肽S转移酶 表面等离子体子谐振 SPR 特异性 改良 |
| 收稿时间： | 2006-09-01 |
Improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip |
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| Li Hua CHEN. Improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip[J]. Chinese Chemical Letters, 2006, 17(12): 1619-1622 |
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| Authors: | Li Hua CHEN |
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| Abstract: | A chip was modified with bovine serum albumin (BSA), then interaction between glutathione (GSH) immobilized on the top of BSA and glutathione-S-transferase (GST) was examined, using surface plasmon resonance (SPR). The SPR results showed that BSA-modified chip was effective not only in binding the target proteins but also in suppressing the nonspecific binding (NSB) of proteins. |
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| Keywords: | Surface plasmon resonance (SPR) bovine serum albumin (BSA) glutathione (GSH) glutathione-S-transferase (GST). |
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