| DNA G-四链体的结构完整性对催化性质的影响 |
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| 引用本文： | 陈杰林,程明攀,王佳伟,仇得辉,David Monchaud,Jean-Louis Mergny,鞠熀先,周俊. DNA G-四链体的结构完整性对催化性质的影响[J]. 催化学报, 2021, 42(7): 1102-1107,中插7-中插11. DOI: 10.1016/S1872-2067(20)63744-5 |
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| 作者姓名： | 陈杰林 程明攀 王佳伟 仇得辉 David Monchaud Jean-Louis Mergny 鞠熀先 周俊 |
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| 作者单位： | 南京大学化学化工学院, 生命分析化学国家重点实验室, 江苏南京210023, 中国;勃艮第大学分子化学研究所, CNRS UMR6302, 第戎21000, 法国;南京大学化学化工学院, 生命分析化学国家重点实验室, 江苏南京210023, 中国;巴黎综合理工学院, 光学与生物科学实验室, CNRS, INSERM, 帕莱索91128, 法国 |
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| 基金项目： | Fundamental Research Funds for the Central Universities ;This work was supported by the National Natural Science Foundation of China ;中央高校基本科研业务费;南京大学高层次人才科研启动经费;国家自然科学基金;the Funds of Nanjing University |
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| 摘 要： | DNA酶中的G-四链体-血红素(G4-hemin)DNA酶结构具有较高的设计性和化学稳定性,因此格外受研究者关注.G-平面作为辅酶因子hemin的结合位点,不仅提供大π平面与hemin结合,而且其平面上的G碱基还可以充当近端配位基团与hemin进行配位.因此,研究G-平面完整性在G4-DNA酶体系中的作用具有重要意义....
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| 关 键 词： | G-四链体 G-四链体DNA酶 G-空位 G-平面完整性 鸟嘌呤衍生物 |
The catalytic properties of DNA G-quadruplexes rely on their structural integrity |
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| Jielin Chen,Mingpan Cheng,Jiawei Wang,Dehui Qiu,David Monchaud,Jean-Louis Mergny,Huangxian Ju,Jun Zhou. The catalytic properties of DNA G-quadruplexes rely on their structural integrity[J]. Chinese Journal of Catalysis, 2021, 42(7): 1102-1107,中插7-中插11. DOI: 10.1016/S1872-2067(20)63744-5 |
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| Authors: | Jielin Chen Mingpan Cheng Jiawei Wang Dehui Qiu David Monchaud Jean-Louis Mergny Huangxian Ju Jun Zhou |
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| Affiliation: | (State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,Jiangsu,China;Institut de Chimie Moléculaire,Universitéde Bourgogne(ICMUB),CNRS UMR6302,UBFC Dijon 21000,France;Laboratoire d’Optique et Biosciences,Ecole Polytechnique,CNRS,INSERM,Institut Polytechnique de Paris,91128 Palaiseau,France) |
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| Abstract: | The influence of the G-quartet structural integrity on the catalytic activity of the G-quadruplex (G4) was investigated by comparing the G4-DNAzyme performances of a series of G4s with a G-vacancy site and a G-triplex (G-tri). The results presented herein not only confirm that the structural integ-rity of the 3'-end G-quartet is necessary for G4s to be catalytically competent but also show how to remediate G-vacancy-mediated catalytic activity losses via the addition of guanine surrogates in an approach referred to as G-vacancy complementation strategy that is applicable to parallel G4s only. Furthermore, this study demonstrates that the terminal G-quartet could act as a proximal coordi-nating group and cooperate with the flanking nucleotide to activate the hemin cofactor. |
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| Keywords: | G-quadruplex G-quadruplex DNAzyme G-vacancy G-quartet integrity Guanine surrogate |
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