| 气相色谱-氮磷检测法检测尿中劳拉西泮 |
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| 引用本文： | 姜兆林,谭家镒,姚丽娟,邢丽梅.气相色谱-氮磷检测法检测尿中劳拉西泮[J].色谱,2001,19(4):341-343. |
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| 作者姓名： | 姜兆林 谭家镒 姚丽娟 邢丽梅 |
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| 作者单位： | 中国刑警学院法医系, |
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| 基金项目： | 国家“九五”攻关子专题(96-919-01-05-4) |
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| 摘 要： | 报道了尿中劳拉西泮的气相色谱 氮磷检测器的检测方法。检测时以 2 羟乙基氟西泮为内标 ,用 β 葡萄糖醛酸苷酶水解后于碱性条件下用乙醚萃取 ,将萃取液浓缩后进行检测。劳拉西泮的萃取率 (mean±SD)为 ( 83 4±3 1) % ,检出限为 5 μg/L。萃取物用N ,O 双 (三甲硅烷基 )三氟乙酰胺 (BSTFA)衍生化后进行三甲基硅烷 (TMS)衍生物检测。
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| 关 键 词： | 气相色谱法 劳拉西泮 尿 |
| 文章编号： | 1000-8713(2001)04-0341-03 |
| 修稿时间： | 2000年11月6日 |
The Determination of Lorazepam in Human Urine by Gas Chromatography/Nitrogen-Phosphorus Detector |
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| JIANG Zhao lin,\ TAN Jia yi,\ YAO Li juan,\ XING Li mei.The Determination of Lorazepam in Human Urine by Gas Chromatography/Nitrogen-Phosphorus Detector[J].Chinese Journal of Chromatography,2001,19(4):341-343. |
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| Authors: | JIANG Zhao lin \ TAN Jia yi \ YAO Li juan \ XING Li mei |
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| Institution: | Dept. of Forensic Medicine, China Criminal Police College, Shenyang 110035, China. |
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| Abstract: | A method to assay lorazepam in human urine has been developed. After addition of hydroxyethylflurazepam (internal standard) and hydrolysis with beta-glucuronidase, the lorazepam and hydroxyethylflurazepam were extracted with ethyl ether at pH 10.8. The analysis was performed on an HP-5 capillary column with nitrogen-phosphorus detector(NPD). The detection limit and recovery of analytes in urine were 5 micrograms/L and (83.4 +/- 3.1)% respectively. The method was successfully applied to urine specimens collected from healthy human volunteers who have ingested 2 mg of lorazepam. The method was sensitive enough to assay urine specimen excreted at 32 h after taking the medicine by volunteers. |
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| Keywords: | gas chromatography lorazepam urine |
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