Effect of DNA and bivalent metal ions on the interaction of thermostable DNA polymerase <Emphasis Type="Italic">Tte</Emphasis> with dNTPs |
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| Authors: | E A Belousova V V Koval N I Rechkunova S Kh Degtyarev O S Fedorova O I Lavrik |
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| Institution: | (1) Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 8 prosp. Akad. Lavrent'eva, 630090 Novosibirsk, Russian Federation;(2) Institute of Molecular Biology and Biophysics, Siberian Branch, Russian Academy of Medical Sciences, 2 ul. Timakova, 630117 Novosibirsk, Russian Federation |
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| Abstract: | The interaction of DNA polymerase Tte from Thermus thermophilus B35 with dUTP analog containing a fluorescein residue bound to C(5) of the base (Flu-dUTP) was studied by fluorescence titration.
The dissociation constants of the enzyme—substrate complexes in the absence and in the presence of a DNA duplex containing
an extended template and bivalent metal ions and the kinetic parameters of polymerization by DNA polymerase Tte in the presence of Flu-dUTP were determined.
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Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 1268–1272, May, 2005. |
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| Keywords: | DNA replication fidelity of DNA synthesis thermostable DNA polymerase Tte fluorescent derivatives of dNTP |
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