Neuropeptide imaging on an LTQ with vMALDI source: The complete ‘all-in-one’ peptidome analysis |
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| Authors: | Peter D Verhaert Maria C Prieto Conaway Tonya M Pekar Ken Miller |
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| Institution: | aAnalytical Biotechnology Laboratory, Delft University of Technology, Delft, The Netherlands;bBioMedical Research Center, University of Hasselt, Belgium;cMass Spectrometry Laboratory, University of Liège, Belgium;dFlemish Interuniversity Institute of Biotechnology, Molecular Cell Biology Laboratory, Leuven, Belgium;eThermo Electron Corporation, San Jose, CA, USA |
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| Abstract: | Direct tissue imaging was performed on dissected insect tissue using a MALDI ion trap to visualize endogenous neuropeptides. Coupling tissue imaging to tandem MSn allows for the identification of previously known species and the ability to identify new ones by de novo sequencing, as searchable databases for insects are sparse. Direct tissue imaging is an attractive technique for the study of neuropeptides as minimal sample preparation is required prior to mass spectrometry. We successfully identified neuropeptides present in the corpora cardiaca and allata of Acheta domesticus (the house cricket). Diagnostic fragments at low m/z were used to distinguish between lipids and neuropeptides. The distribution of peptides appears to be more differentially localized than that of phospholipids, which seem to be more evenly distributed within the tissue. |
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| Keywords: | MALDI MS imaging Ion trap MSn Neuropeptide |
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