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A Fully Automatic HPLC–CAD–DAD Method Coupled with ASE and Online SPE for Simultaneous Determination of Seven Antibiotics in Bio-Matrices |
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| Authors: | Zhou Qian Wang Nan Zhu Lihua Tang Heqing |
| Institution: | 1.College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China ;2.Key Laboratory of Catalysis and Materials Science of the State Ethnic Affairs Commission and Ministry of Education, College of Resources and Environmental Science, South-Central University for Nationalities, Wuhan, 430074, People’s Republic of China ; |
| Abstract: | It is a challenge to simultaneously determine the multiple-component targets in complex matrices when some of the targets bear chromophores but some contain no chromophores. A novel HPLC–CAD–DAD method coupled with accelerated solvent extraction and online solid phase extraction (SPE) was developed for simultaneous determination of seven antibiotics in solid bio-matrices. The online SPE operation parameters were optimized on a Acclaim®120 C18 cartridge in terms of extraction efficiency with sample injection volume of 1 mL, and the online SPE operation yielded enhancement factors in the range of 29–77 for the different antibiotics. The proposed method showed good recoveries ranging between 79.2 and 104.7 % for different antibiotics and excellent inter-day and intra-day precision with relative standard deviation values ranging from 0.6 to 7.8 %. The detection limits were between 1.8 and 18.0 μg kg−1. |
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