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An Optochemical Oxygen Scavenger Enabling Spatiotemporal Control of Hypoxia |
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| Authors: | Naoya Ieda Masato Sawada Runa Oguchi Masato Itoh Seina Hirakata Daisuke Saitoh Akito Nakao Mitsuyasu Kawaguchi Prof Kazunobu Sawamoto Prof Toshitada Yoshihara Prof Yasuo Mori Prof Hidehiko Nakagawa |
| Institution: | 1. Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya-shi, Aichi, 467-8603 Japan;2. Graduate School of Medical Sciences, Nagoya City University, 1, Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya-shi, Aichi, 467-8601 Japan;3. Graduate School of Science and Technology, Gunma University, 1-5-1, Tenjin-cho, Kiryu-shi, Gunma, 376-8515 Japan;4. Graduate School of Engineering, Kyoto University Katsura, Katsura, Nishikyo-ku, Kyoto, 615-8510 Japan |
| Abstract: | We present an optochemical O2 scavenging system that enables precise spatiotemporal control of the level of hypoxia in living cells simply by adjusting the light intensity in the illuminated region. The system employs rhodamine containing a selenium or tellurium atom as an optochemical oxygen scavenger that rapidly consumes O2 by photochemical reaction with glutathione as a coreductant upon visible light irradiation (560–590 nm) and has a rapid response time, within a few minutes. The glutathione-consuming quantum yields of the system were calculated as about 5 %. The spatiotemporal O2 consuming in cultured cells was visualized with a hypoxia-responsive fluorescence probe, MAR. Phosphorescence lifetime imaging was applied to confirmed that different light intensities could generate different levels of hypoxia. To illustrate the potential utility of this system for hypoxia research, we show that it can spatiotemporally control calcium ion (Ca2+) influx into HEK293T cells expressing the hypoxia-responsive Ca2+ channel TRPA1. |
| Keywords: | Hypoxia Oxygen Scavenger Photocontrol TRPA1 |