| 苎麻叶中绿原酸的分光光度法测定 |
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| 引用本文： | 李勃,瞿龙,施树云.苎麻叶中绿原酸的分光光度法测定[J].广州化学,2005,30(2):41-44. |
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| 作者姓名： | 李勃 瞿龙 施树云 |
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| 作者单位： | 1. 中南大学,化学化工学院,湖南,长沙 410083
2. 浙江大学,药学院,浙江,杭州,310027 |
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| 摘 要： | 探讨了采用分光光度法测定苎麻叶中绿原酸的含量的方法。12.5%的醋酸用量为0.1mL,7%尿素2.0mL,0.5%亚硝酸钠0.25mL,3min后加入5%氢氧化钠0.5mL,绿原酸与试剂形成鲜红色络合物,通过在510nm处测定溶液的吸光度确定绿原酸的含量。绿原酸浓度在0.01~0.12g/L范围内与吸光度值有良好的线性关系,回收率为95%~107%,相对标准偏差(RSD)为2.4%。该方法简便、快速。
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| 关 键 词： | 苎麻叶 绿原酸 分光光度法 |
| 文章编号： | 1009-220X(2005)02-0041-04 |
| 修稿时间： | 2004年7月11日 |
Spectrophotometric Determination of Chlorogenic Acid in Leaves of Ramie |
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| LI Bo,QU Long,SHI Shu-yun.Spectrophotometric Determination of Chlorogenic Acid in Leaves of Ramie[J].Guangzhou Chemistry,2005,30(2):41-44. |
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| Authors: | LI Bo QU Long SHI Shu-yun |
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| Institution: | LI Bo1,QU Long1,SHI Shu-yun2 |
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| Abstract: | A method for the spectrophotometric determination of chlorogenic acid in leaves of ramie has been established. The additive amount of acetic acid(12.5%)is 0.1 mL, urea(7%)and sodium nitrite(0.5%)is 2 mL and 0.25 mL respectively. Then 0.5 mL sodium hydroxide(0.5%) was added 3 minutes later. At that time, the red complex was formed, and then chlorogenic acid was determined by spectrophotometer at 510 nm. The linear range is 0.01 ~ 0.12 g/L with a correlation coefficient of 0.9998. The recovery and RSD are 95% ~ 107% and 2.4% respectively. This method is simple and rapid. |
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| Keywords: | leaves of ramie chlorogenic acid spectrophotometry |
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