| DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM PROTOPLAST-DERIVED CELLS OF WHEAT (Triticum aestivum L.) |
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| 作者姓名： | 郭光沁 夏光敏 李忠谊 陈惠民 |
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| 作者单位： | Biology Department，Shandong University，Jinan 250100，PRC，Biology Department，Shandong University，Jinan 250100，PRC，Biology Department，Shandong University，Jinan 250100，PRC，Biology Department，Shandong University，Jinan 250100，PRC |
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| 摘 要： | Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB mediumsupplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cellsfrom protoplasts divided to form somatic embryos directly. The somatic embryos grown to1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor-mones.
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DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM PROTOPLAST-DERIVED CELLS OF WHEAT (Triticum aestivum L.) |
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| GUO GUANG-QIN,XIA GUANG-MIN,LI ZHONG-YI,CHEN HUI-MIN Biology.DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM PROTOPLAST-DERIVED CELLS OF WHEAT (Triticum aestivum L.)[J].Science in China(Chemistry),1991(4). |
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| Authors: | GUO GUANG-QIN XIA GUANG-MIN LI ZHONG-YI CHEN HUI-MIN Biology |
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| Institution: | GUO GUANG-QIN,XIA GUANG-MIN,LI ZHONG-YI,CHEN HUI-MIN Biology Department,Shandong University,Jinan 250100,PRC |
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| Abstract: | Protoplasts were isolated from embryogenic calli derived from immature embryos of
wheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB medium
supplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cells
from protoplasts divided to form somatic embryos directly. The somatic embryos grown to
1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor-
mones. |
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| Keywords: | wheat (Triticum aestivum L ) protoplast direct somatic embryogenesis plantlet regeneration |
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