尿毒症患者血清中中分子物质的提取及性质的初步表征

从尿毒症患者血清及腹透液中得到的中分子量物质,具有相同的凝胶色谱行为及紫外、红外吸收行为,它们在206和235nm处,有特征紫外吸收峰;在波数为3246,1516,1122和613cm~(-1)处,有特征红外吸收峰。中分子物经离子交换色谱进一步分离,发现起决定紫外及红外吸收行为的成分集中于第3子峰;其自由氨基酸和肽类物质含量均低于3％。

Isolation and Preliminary Characterization of Properties of Middle Molecules from Sera of Uremic Patients

Middle molecules in sera of uremic patients (UMM) were isolated on Sephadex G-15 gel-filtration Chromatography eluted with triethylamine caibonate buffer. Dry sample of middle molecules could be obtained with lyophilization. From the dialysis fluids of the uremic patients treated with continuous ambulatory peritoneal dialysis (CAPD) the same UMM fractions could be obtained. The behaviours of gel-filtration chromatography, UV and JR absorptions of UMM from the sera and the dialysis fluids of uremic patients were identical. In 206 nm and 235 nm, there were 2 UV absorption peaks and in 3 246, 1 516, 1 122 and 613 cm-1 there were 4 characteristic IR- absorption peaks which are not found in normal sera. The Compositions responsible for the behaviours of UV and IR absorptions are found in the third sub-peak after the middle molecules were further separated by ion-exchange chromatography. The contents of peptides and free amino acid from the sample of UMM were quantitatively analyzed. They are between one to three percent.