| 改进溶胶—凝胶法固定酶结构剖析及在苯酚光化学传感器？… |
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| 引用本文： | 李军,王柯敏.改进溶胶—凝胶法固定酶结构剖析及在苯酚光化学传感器？…[J].高等学校化学学报,2000,21(7):1018-1022. |
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| 作者姓名： | 李军 王柯敏 |
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| 作者单位： | 湖南大学化学化工学院!长沙410082 |
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| 基金项目： | 国家自然科学基金! (批准号 :2 96750 0 5)，国家杰出青年基金! (批准号 :2 982 5110 )资助 |
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| 摘 要： | 通过光学显微镜比较了用密闭老化法和浸涂法制备的溶胶－凝胶膜的表面状态，从实验上证实了用密闭老化法制备的溶胶－凝胶膜不会破裂，并用透射电子显微镜了包埋根过氧化物酶的溶胶－凝胶膜的内部结构。
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| 关 键 词： | 苯酚 光化学传感器 溶胶－凝胶膜 酶固定技术 |
The Microstructure Analysis of Enzymes Immobilized by Sol-gel Method and Its Application in Phenol Optical Chemical Sensor |
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| LI Jun,WANG Ke Min ,XIAO Dan,YANG Xiao Hai.The Microstructure Analysis of Enzymes Immobilized by Sol-gel Method and Its Application in Phenol Optical Chemical Sensor[J].Chemical Research In Chinese Universities,2000,21(7):1018-1022. |
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| Authors: | LI Jun WANG Ke Min XIAO Dan YANG Xiao Hai |
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| Institution: | LI Jun,WANG Ke Min *,XIAO Dan,YANG Xiao Hai |
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| Abstract: | The external states of horseradish peroxidase(HRP) encapsulated membrane prepared by enclosed aging method and dip coating method are compared by common optical microscopy to clarify that the sol gel membrane prepared by enclosed aging method is no cracking. The transmission electron microscopy(TEM) is used to observe the character of the sol gel derived HRP membrane. The TEM photos show that enzyme can uniformly distribute in the gel as if in the solution and do not leach from the gel. This membrane can be used to fabricate a phenol optical sensor based on the decrease of chemiluminescence intensity. The response mechanism is discussed by compete reaction. |
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| Keywords: | Sol gel Horseradish peroxidase Microstructure Phenol Optical sensor |
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