GC-MS Identification and GC-FID Quantitation of Terpenoids in Ononidis spinosae Radix |
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Authors: | Daruhzi gnes E Szarka Szabolcs Hthelyi va Simndi Bla Gyurjn Istvn Lszl Mikls Szke va Lemberkovics va |
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Institution: | 1.Department of Pharmacognosy, Semmelweis University, Üllői Str. 26, 1085, Budapest, Hungary ;2.Department of Chemical Engineering, University of Technology and Economics, Műegyetem rkp. 3, 1111, Budapest, Hungary ;3.Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter Sétány 1/C, 1117, Budapest, Hungary ; |
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Abstract: | A GC-MS method has been developed for the qualitative analysis of sterol and triterpene (terpenoid) constituents of Ononis spinosa L. (spiny restharrow) root without derivatization. β-Sitosterol, campesterol, stigmasterol, stigmastan-3,5-diene sterol compounds and the triterpene derivatives β-amyrin and α-onocerin were identified. A validated GC-FID quantitative method was developed for measuring β-sitosterol, the main sterol component, in various extracts of this plant, obtained with organic solvents and by supercritical fluid extraction. The extracts were cleaned by saponifying and then the β-sitosterol was quantified in the non-saponifiable fractions by GC-FID with an internal standard. In addition, the relative concentrations of the other terpenoids were also determined. The β-sitosterol content in the non-saponifiable solvent extraction fractions was 0.19–5.5%, that of the supercritical fractions were 4.8–9.2%, depending on the experimental conditions. The hexane and the pilot scale SFE extracts were considered as main sources of terpenoids (71.8%; 93.3%, respectively). |
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