Spectroscopic properties of DNA-acridine orange biopolymer films

Using electronic absorption, luminescence, and vibrational spectroscopy, we study the properties of DNA-acridine orange biopolymer films. The conformational structure of DNA molecules in the film is examined at different levels of the relative humidity, and the state of dye molecules incorporated into the structure of the biopolymer is estimated. These structures were formed according to the “guest-host” principle and the dye molecule acts as the “guest” and the “host” is the intercalative DNA binding sites. In such system dye molecules demonstrate high luminescence capability and are the uniform optical centers and their fluorescence spectrum doesn’t depend on excitation wavelength.