| 流动注射化学发光法测定兽药制剂中恩诺沙星 |
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| 引用本文： | 孙汉文,汪飞,陈培云. 流动注射化学发光法测定兽药制剂中恩诺沙星[J]. 分析试验室, 2009, 28(12) |
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| 作者姓名： | 孙汉文 汪飞 陈培云 |
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| 作者单位： | 河北大学化学与环境科学学院,河北省分析科学技术实验室,保定,071002 |
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| 基金项目： | 教育部博士点学科专项基金和河北省自然科学基金 |
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| 摘 要： | 建立了流动注射化学发光检测恩诺沙星的新方法.考察和优化了影响KMnO_4-Na_2S_2O_3-恩诺沙星体系化学发光强度的条件,通过比较荧光光谱和化学发光光谱探讨了化学发光机理.在3.0×10~(-7)～3.0×10~(-6) g/mL范围内,化学发光强度与恩诺沙星的浓度之间呈现良好的线性关系.检出限为1.1×10~(-7) g/mL,相对标准偏差为2.7%.本方法已用于兽药制剂中恩诺沙星含量的测定.
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| 关 键 词： | 流动注射 化学发光 恩诺沙星 兽药制剂 |
Flow-injection chemiluminescence method for the determination of fnrofloxacin in veterinary preparations |
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| SUN Han-wen,WANG Fei,CHEN Pei-yun. Flow-injection chemiluminescence method for the determination of fnrofloxacin in veterinary preparations[J]. Chinese Journal of Analysis Laboratory, 2009, 28(12) |
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| Authors: | SUN Han-wen WANG Fei CHEN Pei-yun |
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| Affiliation: | SUN Han-wen,WANG Fei and CHEN Pei-yun(College of Chemistry and Environmental Science,Hebei University,Key Laboratory of Analytical Science and Technology of Hebei Province,Baoding 071002) |
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| Abstract: | A novel flow-injection chemiluminescence method was developed for the determination of enrofloxacin with KMnO_4-Na_2S_2O_3-enrofloxacin system. The CL conditions of the system were investigated and optimized. The chemilu-minescence mechanism was discussed by comparison of fluorescence and chemiluminescence spectra. The relationship between chemiluminescence intensity and enrofloxacin concentration was linear in the range of 3.0 × 10~(-7)～3.0 × 10~(-6) g/mL. The detection limit for enrofloxacin was 1.1 × 10~(-7) g/mL, and the relative standard deviation (n = 11) is 2.7% for 3 × 10~(-7) g/mL enrofloxacin. The proposed method has been used for the determination of enrofloxacin in veterinary preparations. |
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| Keywords: | Flow injection Chemiluminescence Enrofloxacin Veterinary preparations |
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