PHOTOSENSITIZING PROPERTIES OF BACTERIOCHLOROPHYLLIN a and BACTERIOCHLORIN a, TWO DERIVATIVES OF BACTERIOCHLOROPHYLL a |
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| Authors: | Eva M Beems Tom M A R Dubbelman Johan Lugtenburg Jaap A van Best Monique F M A Smeets J Pierre J Boegheim |
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| Institution: | Department of Ophthalmology, Leiden University Medical Center, Leiden University, Leiden, The Netherlands;Department of Medical Biochemistry, Sylvius Laboratories, Leiden University, Leiden, The Netherlands;Department of Organic Chemistry, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands |
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| Abstract: | Abstract The photosensitizing properties of two water soluble derivatives of bacteriochlorophyll a , bacteriochlorophyllin a and bacteriochlorin a (lacking the central Mg-ion) were investigated and compared to those of hematoporphyrin derivatives. At physiological pH the oxygen consumption rate of histidine, tryptophan, dithiothreitol and guanosine upon illumination was 3 to 4 times higher when bacteriochlorin a was used as photosensitizer than when hematoporphyrin derivatives were used. Especially bacteriochlorin a proved to be an effective sensitizer for the killing of L929 cells. Because bacteriochlorin a has an absorption maximum at 765 nm in phosphate buffered saline (allowing a light penetration in tissue about ten times larger than at 630 nm) and a high molar absorption coefficient (32 000 M cm−1) it has promising possibilities for the application in photodynamic therapy. |
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