一种新的标定体积排除色谱（SEC）的方法

基于体积排除色谱中测得的淋出体积和动态激光光散射中测得的平动扩散系数都直接依赖于高分子的流体力学体积这一事实，本文在理论上提出了一种把淋出体积分布和平动扩散系数分布二者结合起来标定体积排除色谱的新方法，并且在实验上通过对宽分布的聚苯乙烯标准样品的测试证实了该方法的可行性．

A NOVEL METHOD TO CALIBRATE SIZE EXCLUSION CHROMATOGRAPHY

conventional calibration of size exclusion chromatography (SEC) requires a set of narrowly distributed polymer standards with different molecular weights (M). In this study, a novel method was proposed, wherein the intrinsic connection between the measured retention volume (V) in SEC and the diffusion coefficient (D) in dynamic laser light scattering (LLS), i.e., both v and D are related to the same hydrodynamic volume, is utilized to calibrate both SEC and DLS in a single process by using only ONE broadly distributed polymer sample. One broadly distributed polymer standard (NBS706) was used in this study to verify this proposed method. The calibration between v and M from this method is fairly comparable to that from the conventional method, and the calibration between D and M is also in agreement with the literature values. With these two calibrations, both of the conversions from the retention volume distribution in SEC and diffusion coefficient distribution luG(D) to molecular weight distribution have been accomplished. This novel method will enable us to use SEC to characterize special polymers where the conventional SEC calibration is not applicable.