Gradient elution chromatography of polymers on reversed-phase columns with tetrahydrofuran as an eluent component

Summary The chromatographic separation of poly(styrene-co-acrylonitrile) samples (SAN) with an elution gradient iso-octane/tetrahydrofuran is solubility governed. This has been proven by the correspondence between the volume fraction of precipitant as estimated from elution data and as measured by turbidimetric titration, by the molar mass dependence of retention, and by the temperature effect on retention. In addition to these arguments data are presented which have been obtained on columns with different packing materials ranging from bare silica through CN bonded phase material to hydrocarbonaceous stationary phases. The eluent composition at peak position is nearly independent of the stationary phase used. Presented at the 15th International Symposium on Chromatography, Nürnberg, October 1984