三维荧光光谱结合交替三线性分解二阶校正法同时测定血浆样中三种抗癌药物含量

姜黄素、阿霉素和白藜芦醇对多种恶性肿瘤的治愈皆有一定的疗效，且其联合用药对癌细胞的抑制效果更加显著．本文采用三维激发发射荧光光谱结合基于交替三线性分解（Alternating Trilinear Decomposition，ATLD）算法的化学计量学二阶校正法，同时对血浆样中姜黄素、盐酸阿霉素和白藜芦醇的含量进行了定量分析研究．当量测体系的组分数预估计取4时，ATLD方法解析得到的血浆样中姜黄素、盐酸阿霉素和白藜芦醇的平均回收率分别为（91．4±1．1）％、（104．5±2．8）％和（103．4±4．7）％．实验结果表明，此法能够解决干扰共存和荧光光谱重叠下血浆样中姜黄素、盐酸阿霉素和白藜芦醇的直接、快速、同时定量分析的难题．

Simultaneous determination of three anticancerogens in plasma samples using second-order calibration method based on alternating trilinear decomposition coupled with fluorescence spectroscopy

Curcumin, adriamycin and resveratrol are effective for curing many kinds of cancer, and combination of them can contribute to the prevention of cancer ceils more notably. In this paper, curcumin, adriamycin and resveratrol were determined in plasma samples simultaneously by using three-way excitation-emission fluorescence spectra with second-order calibration in chemometrics based on the alternating trilinear decomposition （ATLD） algorithm. When the estimated number of components was set to 4, the obtained average recoveries of curcumin, adriamycin hydrochloride and resveratrol in plasma samples were （91.4±1.1）%, （104.5±2.8）% and （103.42±4.67）% for ATLD, respectively. The experiment results indicated that the proposed method could be applied to simultaneously quantify the curcumin, adriamycin hydrochloride and resveratrol contents in plasma samples even in the presence of unknown interferences.