| 牛血清白蛋白在NVP/HEMA无规共聚物水凝胶上的吸附研究 |
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| 引用本文： | 谭帼馨,崔英德,肖楚民.牛血清白蛋白在NVP/HEMA无规共聚物水凝胶上的吸附研究[J].离子交换与吸附,2004,20(3):199-204. |
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| 作者姓名： | 谭帼馨 崔英德 肖楚民 |
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| 作者单位： | 1. 广东工业大学轻工化工学院,广州,510080
2. 仲恺农业技术学院,广州,510225 |
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| 基金项目： | 国家自然科学基金资助项目 (29976007)，广东工业大学青年基金资助项目 (012025) |
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| 摘 要： | 合成了不同含水量的N-乙烯吡咯烷酮(NVP)／甲基丙烯酸-β-羟乙酯(HEMA)共聚物水凝胶．研究了温度、pH值，蛋白质初浓度及离子强度等因素对牛血清白蛋白吸附性能的影响．采用紫外分光光度法测定BSA的吸附量．结果表明，蛋白质初浓度越高．温度越高，离子强度越大；蛋白质内部更多的疏水性氧基酸残基暴露于外部．吸附量增加：在等电点pH=4．7附近蛋白质沉积量最大．
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| 关 键 词： | 蛋白质 吸附 水凝胶 NVP HEMA 吸附量 |
| 文章编号： | 1001-5493(2004)03-0199-06 |
| 修稿时间： | 2003年8月31日 |
STUDY ON ADSORPTION OF BOVINE SERUM ALBUMIN ON NVP/HEMA COPOLYMER HYDROGELS |
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| TAN Guoxin CUI Yingde XIAO Chuimin Faculty of Chemical Engineering and Light Industry Guangdong University of Technology,Guangzhou ,China Zhongkai Agrotechnical College,Guangzhou ,China.STUDY ON ADSORPTION OF BOVINE SERUM ALBUMIN ON NVP/HEMA COPOLYMER HYDROGELS[J].Ion Exchange and Adsorption,2004,20(3):199-204. |
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| Authors: | TAN Guoxin CUI Yingde XIAO Chuimin Faculty of Chemical Engineering and Light Industry Guangdong University of Technology Guangzhou China Zhongkai Agrotechnical College Guangzhou China |
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| Institution: | TAN Guoxin1 CUI Yingde2 XIAO Chuimin1 1 Faculty of Chemical Engineering and Light Industry Guangdong University of Technology,Guangzhou 510080,China 2 Zhongkai Agrotechnical College,Guangzhou 510025,China |
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| Abstract: | Hydrogel material was synthesized by copolymerization of NVP and HEMA. The influence of temperature, pH, initial protein concentration and ionic intensity were investigated in detail. The absorbency of BSA was measured with the ultraviolet spectrophotometer. The result showed that the absorption of BSA on NVP/HEMA hydrogels increased with the initial concentration, temperature and ionic intensity increasing. The maximum absorption was observed when the pH of solution was near the isoelectric point of BSA. |
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| Keywords: | Protein Adsorption Hydrogel NVP HEMA Absorbency |
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