Fluorescence Polarization as a Functional Parameter in Monitoring Living Cells: Theory and Practice |
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| Authors: | Mordechai Deutsch Reuven Tirosh Menachem Kaufman Naomi Zurgil Arye Weinreb |
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| Institution: | (1) The Biophysical Interdisciplinary Center for the Research and Technology of the Cellome, and The Jerome Schottenstein Center for Early Detection of Cancer, Bar-Ilan University Ramat-Gan, Israel, 52900 |
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| Abstract: | The use of fluorescence polarization as a functional parameter in monitoring cellular activation calls for the reliable and accurate measurement of the fluorescence intensity and polarization (FI and FP) of microscopic objects. The relevant experimental parameters that enter such measurements are thoroughly discussed. The possibility of executing FP measurements properly by flow-through systems is compared with that of static cytometry. Remarks on the effects of high-power excitation on markers and cells conclude the paper. |
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| Keywords: | Fluorescence polarization flow cytometry static cytometry excitation power density photodamage photo biostimulation individual living cells |
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